Weight loss and gain

All the groups of mice experienced an increase in weight over time.  However, the mice exposed to intermittent fasting were lighter than mice on a controlled (regular) diet, “since they had been on corresponding feeding protocols for 37 weeks.” 

Subsequently, mice on the high-fat diet were heavier than mice on a controlled diet “since they were on corresponding feeding protocol for 13 weeks.”  In the end, “mice on the high-fat diet were 45% heavier than the control mice.”  The conclusion for this observation was that the high-fat diet “significantly” affected the mice’s body weight in a negative way.

Brain Improvements and Impairments

The researchers concluded that intermittent fasting improves learning and memory.  Multifold paradigms and parameters were used to measure the learning, memory, and brain structural impairments and improvements in the mice.  The researchers did point out that “it is still possible that minor brain structural and functional alterations in the obese mice may not be revealed by these methods.” This is obviously a notable variable.

Increased Thickness of the CA1 Pyramidal Cell Layer and Debrin Expression in the Hippocampus

During this study, it was found that there was also an increase in the thickness of the CA1 pyramidal cell layer and debrin expression in the hippocampus of the mice that fasted.  There were no observations suggesting a difference in the mature BDNF expression in the mice that fasted compared to the controlled mice.  “However, intermittent fasting increased the ratio of glutathione/GSSG and reduced HNE and nitrotyrosine containing proteins, two oxidative stress indices, in the cerebral cortex.  These results suggest that intermittent fasting reduces oxidative stress in the brain, since oxidative stress is a known factor change in the expression of oxidative stress indices.”   


To read part 1 of The Brain Improvement Pill click here: Brain Improvement Pill



  1. Li, Liaoliao, and et al. “(PDF) Chronic Intermittent Fasting Improves Cognitive Functions and Brain Structures in Mice.” ResearchGate, 3 June 2013,